A study comparing the oncogenic microRNA-21-5p and the CA15-3 characteristics as an effective tumor marker in breast cancer patients from Iraq.

Breast cancer (BC) is a genetic disease in the mammary glands' ducts and lobules, with ductal cancers comprising most of malignancies. Biomarkers can provide an assessment diagnosis prediction. The study aims to compare expression serum (miR-21-5p) CA 15-3 Iraqi population as more efficient biomarkers, then checked MiRNA-21 main characters biomarker comparison (CA15-3) levels. Circulating miRNA-21 was measured using (the quantitative Real Time-PCR technique) 50 patients at various stages breast compared 27 healthy controls. Meanwhile, levels were quantified electro-chemo luminescence immunoassay (ECLIA) methods. results show concentration CA15-3 increased significantly (p>0.01) control, but higher median level than CA15-3. ROC curve analysis shows that accuracy, Overall Model Quality, AUC, sensitivity specificity much 15-3. In conclusion, may fill gap still lacks detecting early stage. Keywords: cancer, microRNA-21, CA15-3, gene expression, RT-q PCR